USFWS designate critical habitat for the pearl darter (Percina aurora) under the ESA. In total, approximately 524 river miles (843 river kilometers) in Clarke, Covington, Forrest, George, Green, Lauderdale, Jackson, Jones, Newton, Perry, Simpson, Stone, and Wayne Counties, Mississippi, fall within the boundaries of the critical habitat designation. The effect of this regulation is to designate critical habitat for the pearl darter under the Act. This rule is effective 8 May 2023. POC is James Austin, Field Supervisor, U.S. Fish and Wildlife Service, Mississippi Ecological Services Field Office, 6578 Dogwood View Parkway, Jackson, MS 39213; tele: 601–321–1129. (Federal Register 6 April 2023 [Rule] Pages 20410-20430)